Assume you are Dr. Evil, and you wish to attack with a bacterial
based biological weapon. You know that the target is being protected by an antibody based detection system. Your minions are able to steal one of the detection kits and you have access to the antibody being used for detection. Describe a plan you could use to use your bacterial weapon, yet evade the current detection system.
The ability to create genetic code using new nucleotides opens many opportunities to create new proteins with novel amino acids not currently used by life on earth. You have a new amino acid (not one of the ?natural? amino acids used by most life on earth to produce proteins) you would like work with to create a novel protein in E. coli. Describe two biological capabilie that would have to be available in your bacteria to allow the bacteria to ?routinely? produce your desired protein (routine=processes normally associated with current commercial fermentation/protein production and does not require an exceptional level of additional support or addition of external components)
Describe how CRISPR can be used to create a knock out mutation in a particular gene.
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